Evaluation of physiological quality and of vigor in corn seeds after the application of chitosan-based bioproduct

Yasmin Chagas Lima

ORCID iD Universidade Estadual do Ceará (UECE) Brasil

Ana Raquel Lopes Soares de Almeida

ORCID iD Universidade Estadual do Ceará (UECE) Brasil

Silvio Gentil Jacinto Júnior

ORCID iD Universidade Estadual do Ceará (UECE) Brasil

Oriel Herrera Bonilla

ORCID iD Universidade Estadual do Ceará (UECE) Brasil

Eliseu Marlônio Pereira de Lucena

ORCID iD Universidade Estadual do Ceará (UECE) Brasil

Resumo

In favor of a better cultures development and productivity, new studies are seeking different alternatives, efficient technologies, and lower environmental impact to achieve such purposes, thus, the present study aimed to evaluate the application effect of a chitosan-based product in the germination and vigor of corn. The work was carried out at the Ceará State University, Campus Itaperi. A completely randomized design was adopted with four replications of 50 seeds, with the exception of the mass, length tests and image analysis carried out in five replications of 20 seeds. Seeds of the cultivar SM 996 were used, submitted to six concentrations of chitosan-based product: 0.0 (control); 0.5; 1; 2.5; 5, and 10%. It was evaluated the following parameters: first count, germination test, radicle, shoot and seedling length and fresh mass, chlorophyll and carotenoid content, electrolyte extravasation, image computerized analysis by Vigor-S®, emergence, and length, fresh and dry mass of the seedlings in a bed. The use of coating with the bioproduct in corn seeds significantly increased the germination test results, the shoots first count emerged in a bed, as well as, root and seedlings fresh mass, fresh and dry mass in the field, length of the seedlings in the field, seedlings total length and growth index obtained by the Vigor-S®. We concluded that the bioproduct presents as an important bio-stimulant for corn seeds favoring germination parameters, vigor and development of the seedlings.

Palavras-chave


biostimulant; chitosan; coating; germination; Zea mays L.


Texto completo:

Referências


ALFONSO, E. T.; RODRÍGUEZ, A. F.; PADRÓN, J. R.; SOSA, Y. C.; MORALES, H. Agronomic tomato crop response to bioproduct QuitoMax®. Tropical Crops, v. 38, p. 147-154, 2017. DOI: http://dx.doi.org/10.13140/RG.2.2.16534.75843

BRASIL. Ministério da Agricultura, Pecuária e Abastecimento. Regras para análise de sementes. Brasília, DF: MAPA/ACS, 2009. 398 p. Available at: https://www.gov.br/agricultura/pt-br/assuntos/insumos-agropecuarios/arquivos-publicacoes-insumos/2946_regras_analise__sementes.pdf. Accessed on: 20 Oct. 2022. (in Portuguese)

CASSOL, V. M.; FANTINEL, L.; SILVA, W. L. Estudo e viabilidade do revestimento de sementes da soja no processo da germinação a partir do uso de polímero hidrogel de amido de milho. Disciplinarum Scientia, v. 21, n.1, p. 103-115, 2020. DOI: https://doi.org/10.37779/dscnt.v21n1-008. (in Portuguese)

CASTAN, D. O. C.; GOMES-JUNIOR, F. G.; MARCOS-FILHO, J. Vigor-S, a new system for evaluating the physiological potential of maize seeds. Scientia Agricola, v. 75, n. 2, p. 167-172, 2018. DOI: https://doi.org/10.1590/1678-992X-2016-0401.

CHOUDHARY, R. C.; KUMARASWAMY, R. V.; KUMARI, S.; SHARMA, S. S.; PAL, A.; RALIYA, R.; BISWAS, P.; SAHARAN, V. Cu-chitosan nanoparticle boost defense responses and plant growth in maize (Zea mays L.). Representante Científico, v. 7, n. 9754, p. 1-11, 2017. DOI: https://doi.org/10.1038/s41598-017-08571-0.

FERREIRA, G.; BORGHETTI, F. Germinação: do básico ao aplicado. Porto Alegre Artimed, 2004. 324p. (in Portuguese)

FREDDO, Á. R.; MAZARO, S. M; BRUN, E. J.; WAGNER, J. A. Efeito da Quitosana na Emergência, Desenvolvimento Inicial e Caracterização Bioquímica de Plântulas de Acacia mearnsii. Revista Árvore, v. 36, n. 6, p. 1039-1045, 2012. DOI: https://doi.org/10.1590/S0100-67622012000600005. (in Portuguese)

GUAN, Y. J.; HU, J.; WANG, X. J.; SHAO, C. X. Seed conditioning with chitosan improves corn germination and seedling growth in relation to physiological changes under low temperature stress. Journal of Zhejiang University, v. 10, n. 6, p. 427-433, 2009. DOI: https://doi.org/10.1631/jzus.B0820373

KHAN, W. M.; PRITHIVIRAJ, B.; SMITH, D. L. Effect of Foliar Application of Chitin and Chitosan Oligosaccharides on Photosynthesis of Maize and Soybean. Photosynthetica, v. 40, 4, 621-624, 2002. DOI: http://dx.doi.org/10.1023/a:1024320606812.

KRZYZANOWSKI, F.C.; VIEIRA, R. D.; FRANÇA-NETO, J. B.; MARCOS-FILHO, J. Vigor de sementes: conceitos e testes. Londrina: ABRATES, 2020. 601p. (in Portuguese)

LICHTENTHALER, H. K. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol, v. 148, p. 350-381, 1987. DOI: https://doi.org/10.1016/0076-6879(87)48036-1.

LIZÁRRAGA-PAULÍN, E. G.; MIRANDA-CASTRO, S. P.; MORENO-MARTÍNEZ, E.; LARA-SAGAHÓN, A. V.; TORRES-PACHECO, I. Maize seed coatings and seedling sprayings with chitosan and hydrogen peroxide: their influence on some phenological and biochemical behaviors. Journal of Zhejiang University. Science, v. 14, n. 2, p. 87–96, 2013. DOI: https://doi.org/10.1631/jzus.B1200270.

MAHDAVI, B.; RAHIMI, A. Seed priming with chitosan improves the germination and growth performance of ajowan. Eurasian Journal of Biosciences, v. 7, p. 69-76, 2013. DOI: https://doi.org/10.5053/ejobios.2013.7.0.9.

MALERBA, M.; CERANA, R. Recent Advances of Chitosan Applications in Plants. Polymers, v. 10, n. 2, 2018. DOI: https://doi.org/10.3390/polym10020118.

MAKHAYE, G.; MOFOKENG, M. M.; TESFAY, S.; AREMU, A.O.; STADEN, J. V.; AMOO, O. S. Influence of plant biostimulant application on seed germination. In: Biostimulants for Crops from Seed Germination to Plant Development. A Practical Approach, 2021.

MANJUNATHA, G.; ROOPA, K. S.; PRASHANTH, G. N.; SHEKAR SHETTY, H. Chitosan enhances disease resistance in pearl millet against downy mildew caused by Sclerospora graminicola and defence‐related enzyme activation. Pest Management Science: formerly Pesticide Science, v. 64, n. 12, p. 1250-1257, 2008. DOI: https://doi.org/10.1002/ps.1626.

MARTINS, M.; VERONEZE-JUNIOR, V.; CARVALHO, M.; CARVALHO, D. T.; BARBOSA, S.; DORIGUETTO, A. C.; MAGALHAES, P. C.; RIBEIRO, C.; SANTOS, M. H.; SOUZA, T. C. Physicochemical characterization of chitosan and its effects on early growth, cell cycle and root anatomy of transgenic and non-transgenic maize hybrids. Australian Journal of Crop Science, v. 12, n. 1, p. 56-66, 2018. DOI: http://dx.doi.org/10.21475/ ajcs.18.12.01.pne649.

MESA, S. P.; PEDROSO, A. T. R.; ARREBATO, M. R. Efecto de diferentes concentraciones de quitosana sobre la germinación y crecimiento de plántulas de arroz (Oryza sativa, L.). Avances, v. 17, n. 4, p. 380-386, 2015. Available at: https://dialnet.unirioja.es/servlet/articulo?codigo=5350927. Accessed on: 20 Oct. 2022. (in Spanish)

PATERNIANI, E.; CAMPOS, M. S. Melhoramento do milho. In: Borém, A, Melhoramento de espécies cultivadas. Viçosa: Editora UFV, 2005. (in Portuguese)

PEDROSO, A. T. R.; PÉREZ, J. J. R.; MARTÍNEZ, Y. M.; ARREBATO, M. A. R.; RODRÍGUEZ, A. F.; FERNÁNDEZ, Y. V.; MONTIEL, L. G. H. Efecto del Quitomax® en el rendimiento del cultivo de arroz (Oryza sativa L.) var. J-104. Revista de la Facultad de Agronomía de La Universidad del Zulia, v. 36, n. 2, p. 98-110, 2019. (in Spanish)

PEDROSO, A. T. R.; RAMÍREZ-ARREBATO, M. Á.; FALCÓN-RODRÍGUEZ, A.; BAUTISTA-BAÑOS, S.; VENTURA-ZAPATA, E.; VALLE-FERNÁNDEZ, Y. Efecto del Quitomax® en el rendimiento y sus componentes del cultivar de arroz (Oryza sativa L.) var. INCA LP 5. Cultivos Tropicales, v. 38, p. 156-159, 2017. (in Spanish)

PEREIRA, C. E.; OLIVEIRA, J. A.; EVANGELISTA, J. R. E. Qualidade fisiológica de sementes de milho tratadas associadas a polímeros durante o armazenamento. Ciência e Agrotecnologia, v. 29, n. 6, p. 1201-1208, 2005. https://doi.org/10.1590/S1413-70542005000600014. (in Portuguese)

ROLIM, A. E. H.; CARVALHO, F. A. A.; COSTA, R. C. C.; ROSA F. P. Arcabouços de Quitosana: Propriedades Físico-Químicas e Biológicas para o Reparo Ósseo. Revista Virtual de Química, p. 10, n. 2, p. 1-18, 2018. https://doi.org/10.21577/1984-6835.20180018. (in Portuguese)

SAHARAN, V.; KUMARASWAMY, R. V.; CHOUDHARY, R. C.; KUMARI, S.; PAL, A.; RALIYA, R.; BISWAS, P. Cu-chitosan nanoparticle mediated sustainable approach to enhance seedling growth in maize by mobilizing reserved food. Journal of agricultural and food chemistry, v. 64, n. 31, p. 6148-6155, 2016. DOI: https://doi.org/10.1021/acs.jafc.6b02239.

TARHANEN, S.; METSÄRINNE, S.; HOLOPAINEN, T.; OKSANEN, J. Membrane permeability response of lichen Bryoria fuscescens to wet deposited heavy metals and acid rain. Environmental Pollution, v. 104, p. 121-129, 1999. DOI: https://doi.org/10.1016/S0269-7491(98)00157-2.

ZENG, D.; LUO, X. Physiological Effects of Chitosan Coating on Wheat Growth and Activities of Protective Enzyme with Drought Tolerance. Open Journal of Soil Science, v. 2, n. 3, p. 282- 288, 2012. DOI: https://doi.org/10.4236/ojss.2012.23034.

ZERPA, J. A. M.; RINCÓN, M. C.; RINCÓN, D.; COLINA, J. A. V. Efecto del uso de quitosano en el mejoramiento del cultivo del arroz (Oryza sativa L. variedad sd20a). Revista de investigación agraria y ambiental, v. 8, n. 2, p. 151-165, 2017. DOI: https://doi.org/10.22490/21456453.2041. (in Spanish)


DOI: http://dx.doi.org/10.18265/2447-9187a2022id7362

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